產品名稱：（非衍生化 串聯質譜法LC-MS/MS ）
產品型號：57000
產品報價：
產品特點：
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960 analyses
This Chromsystems reagent kit allows the very fast and reliable determination of amino acids and acylcarnitines from dried blood spot samples as part of the newborn screening for amino acid and fatty acid metabolic disorders using tandem mass spectrometry. Sample preparation is based on effective extraction of the analytes from the filter card without any further sample preparation steps required. The test utilises stable isotope labelled internal standards for calibration and measurement to ensure reliable and precise quantification of the analytes. Due to the time saving sample preparation and high precision of the MRM method, this direct injection kit stands out from the derivatised method. On the other hａnd lower sensitivity and interferences from isobaric substances (e.g. C5DC and C6OH) in acylcarnitines analyses may occur.
| Linearity: | Amino acids: 2000-2400 µmol/l (Acyl-)carnitines: 25-200 µmol/l |
| Limit of quantification: | Amino acids: 2.5-30 µmol/l (Acyl-)carnitines: 0.1-1.6 µmol/l |
| Recovery: | Amino acids: 64-94 % (Acyl-)carnitines: 63-102 % |
| Intra-assay: | Amino acids: 2-11 % (Acyl-)carnitines: 3-12 % |
| Inter-assay: | Amino acids: 3.5-18 % (Acyl-)carnitines: 4.8-14 % |
| Analysis time: | < 2 min |
| Amino acids: | ca. 30 to 1000 µmol/l |
| free carnitine: | ca. 55 µmol/l |
| Acylcarnitines: | ca. 0.2 to 10 µmol/l |
LC-MS/MS system. No HPLC column is required.
| Injection volume: | 10 µl |
| Flow gradient: | 20 to 600 µl/min |
| Autosampler rinsing solution: | Rinsing Solution (57007) |
| MS/MS Mode: | MRM |
| Mass transitions: | |
| Alanine | 90 > 44 |
| Alanine-D4 | 94 > 48 |
| Arginine | 175 > 70 |
| Arginine-D7 | 182 > 77 |
| Aspartic acid | 134 > 116 |
| Aspartic acid-D3 | 137 > 119 |
| Citrulline | 176 > 113 |
| Citrulline-D2 | 178 > 115 |
| Glutamic acid | 148 > 130 |
| Glutamic acid-D5 | 153 > 135 |
| Glycine | 76 > 30 |
| Glycine-13C2-15N | 79 > 32 |
| Leucine | 132 > 86 |
| Leucine-D3 | 135 > 89 |
| Methionine | 150 > 133 |
| Methionine-D3 | 153 > 136 |
| Ornithine | 133 > 70 |
| Ornithine-D6 | 139 > 76 |
| Phenylalanine | 166 > 120 |
| Phenylanlanine-D5 | 171 > 125 |
| Proline | 116 > 70 |
| Proline-D7 | 123 > 77 |
| Tyrosine | 182 > 136 |
| Tyrosine-D4 | 186 > 140 |
| Valine | 118 > 72 |
| Valine-D8 | 126 > 80 |
| Free carnitine | 162 >85 |
| Carnitine-D9 | 171 > 85 |
| C2-Carnitine | 204 > 85 |
| C2-Carnitine-D3 | 207 > 85 |
| C3-Carnitine | 218 > 85 |
| C3-Carnitine-D3 | 221 > 85 |
| C4-Carnitine | 232 > 85 |
| C4-Carnitine-D3 | 235 > 85 |
| C5-Carnitine | 246 > 85 |
| C5-Carnitine-D9 | 255 > 85 |
| C5DC-Carnitine | 276 > 85 |
| C5DC-Carnitine-D6 | 282 > 85 |
| C6-Carnitine | 260 > 85 |
| C6-Carnitine-D3 | 263 > 85 |
| C8-Carnitine | 288 > 85 |
| C8-Carnitine-D3 | 291 > 85 |
| C10-Carnitine | 316 > 85 |
| C10-Carnitine-D3 | 319 > 85 |
| C12-Carnitine | 344 > 85 |
| C12-Carnitine-D3 | 347 > 85 |
| C14-Carnitine | 372 > 85 |
| C14-Carnitine-D3 | 375 > 85 |
| C16-Carnitine | 400 > 85 |
| C16-Carnitine-D3 | 403 > 85 |
| C18-Carnitine | 428 > 85 |
| C18-Carnitine-D3 | 431 > 85 |
| Order no. | Product |
|---|---|
| 57000 | MassChrom® Reagent kit for the determination of amino acids and acylcarnitines from dried blood spot (LC-MS/MS)/Non Derivatised with regular 96 well plates. For 960 analyses. |
| 57000/F | MassChrom® Reagent kit for the determination of amino acids and acylcarnitines from dried blood spot (LC-MS/MS)/Non Derivatised with 96 well filter plates. For 960 analyses. |
| Separately available components | |
| 57001 | Mobile Phase, 1000 ml |
| 57002 | Mobile Phase, 10 x 1000 ml |
| 57004/T | Internal Standard (lyoph.), 25 ml |
| 57004 | Internal Standard (lyoph.), 4 x 25 ml |
| 57007 | Rinsing Solution, 1000 ml |
| 57008 | Extraction Buffer, 100 ml |
| 57010 | 96-Well-Plates, V-Boden, 5 pcs. |
| 55011 | Protective sheets for 96 well plates, 10 pcs. |
| 57057 | 96 Well filter plates, 5 pcs. |
| 55013 | Piercable adhesive seal for 96 well plates, 10 pcs. |
| Startup accessories | |
| 55015 | Restrictor Capillary, 1 pc. |
| 55016 | Adapter collar for centrifugation, 2 pcs. |
| 57099 | Tuning Mix, incl. all analytes, 2 ml |
| 55033 | PEEK prefilter, 5 µm, 5 pcs. |
| 15010 | PEEK prefilter housing, 1 pc. |
| Chromsystems quality control material (filter spot) | |
| 0191 | MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Bi-Level (I+II), 2 x 3 Spots |
| 0192 | MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level I, 1 x 3 spots |
| 0193 | MassCheck® Amino Acids, Acylcarnitines Dried Blood Spot Control, Level II, 1 x 3 spots |
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